Effect of cytosine methylation on cutting by the restriction enzyme MaeII
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چکیده
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15 صفحه اولRestriction enzyme HincII is sensitive to methylation of cytosine that occurs 5' to the recognition sequence.
In this paper we demonstrate that the HincII restriction endonuclease, in addition to being sensitive to methylation of the 3′ A and C residues, is also sensitive to methylation of a cytosine immediately 5′ to the recognition sequence. Having encountered this property in one of the sites in the mouse c-fos gene, we confirmed the sensitivity of HincII to the 5′ cytosine methylation in in vitro m...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.5.2335